England will be without Immanuel Feyi-Waboso and Tom Curry for the remainder of their Nations Championship campaign after both players were ruled out due to injury.
It comes as Steve Borthwick's side has been hit with a series of injuries ahead of their clash against Japan on Saturday.
Seb Atkinson, who came off in the second-half of England's 45-22 win against Australia, is set to miss the match with a hip injury, along with Ollie Chessum (shoulder) and George Furbank (groin).
Please use Chrome browser for a more accessible video player
England are optimistic the trio will be available for their clash against New Zealand at Allianz Stadium on Saturday, November 21.
Feyi-Waboso sustained an injury to his right ankle during the first-half of England's clash against the Wallabies, following a hip-drop tackle by Australia prop Angus Bell.
Bell was later sent to the sin bin, with Henry Pollock coming on to replace Feyi-Waboso on the wing at half-time. Pollock would go on to score twice.
The Exeter winger's absence could see Borthwick call on Saracens fliers Noah Caluori or Tobias Elliott for England's clash against Japan.
It would be Caluori's third cap for England, having made his senior debut during their Nations Championship clash against Fiji in July.
Curry did not feature in England's win over Australia after he sustained a hip injury during training last week.
Borthwick revealed after Sunday's win that his brother, Ben Curry, was unlikely to start against Japan, despite the openside enjoying an excellent outing against the Wallabies.
It is likely Borthwick could deploy Freddie Steward at full-back in place of Furbank and keep Marcus Smith on the bench as utility cover.
Please use Chrome browser for a more accessible video player
England's 36-player squad returned to Pennyhill Park on Monday to prepare for this weekend's clash, with Jack Kenningham and Max Hicks both being drafted into the squad as back-row cover for Ben Curry and Chessum.
Meanwhile, Archie McParland has returned to the training squad after recovering from a calf injury.
England training squad
Max Hicks (Newcastle Red Bulls)
Jack van Poortvliet (Leicester Tigers)
Seb Atkinson (Gloucester Rugby), Ollie Chessum (Leicester Tigers), George Furbank (Harlequins)